In addition, LLLT has a pain-relieving effect by reducing the release of arachidonic acid, which reduces prostaglandin E2 levels in the body; by inducing the release of the endogenous opioid neuropeptide beta-endorphin, which exerts a potent analgesic effect; and by stabilising the cell membrane potential, which blocks the activation and transmission of pain-related signals. This shows that LLLT is a promising non-invasive adjunctive intervention for accelerating orthodontic tooth movement.